重组人 Claudin-3蛋白 (去垢剂, His标签)（产品说明）
蛋白名称：Human Claudin-3 Protein (Nanodisc, His Tag)
蛋白构建：A DNA sequence encoding the Human Claudin-3 (O15551) (Met1-Ser183) was expressed with a polyhistidine tag at the C-terminus. This protein was prepared using our Nanodisc platform. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells≥ 80 % as determined by SDS-PAGE.
蛋白活性：Immobilized Recombinant Human Claudin-3 Protein (Nanodisc, His Tag)(Cat: 12196-H08H-NA) at 2 μg/mL (100 μL/well) can bind Anti-CLDN3 Monoclonal Antibody, Human IgG1, the EC50 is 24-72 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant Human Claudin-3 comprises 198 amino acids and has a predicted molecular mass of 20.01 kDa. As a result of glycosylation, the band migrates approximately 20.6 kDa in the SDS-PAGE reducing conditions.
蛋白NP号：O15551
蛋白氨基酸序列：Met1-Ser183