Description：NebuSelect™ Anti-Histidine/His Antibody (Cat# NBAB-210483) was produced in rabbits immunized with 6x His tag synthetic peptide conjugated, and it reacts with all species expected. This antibody is validated for ELISA, IF/ICC, IP, Flow Cytometry, WB.

Reactivity：All Species Expected

Specificity：6x His tagged fusion proteins

Immunogen：6x His tag synthetic peptide conjugated

Isotype：Polyclonal Rabbit IgG

Purification：Protein A Affinity Purification

Buffer：0.2 μm filtered solution in PBS, pH7.4, containing 0.09% Sodium azide, 50% glycerol.

Conjugate：Unconjugated

Applications：ELISA, IF/ICC, IHC, IP, Flow Cytometry, WB

Dilution：ELISA 1:2000～1:10000; IF/ICC 1:50～1:500; IHC 1:50～1:500; IP 1:50～1:500; FC:1:20～1:200; WB: 1:1000～1:5000

Storage：This liquid antibody can be stored at 2℃-8℃ for at least two weeks. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or antibody diluent, the antibody is stable for at least two weeks at 2-4℃.