Description：NebuSelect™ Anti-DYKDDDDK (FLAG® epitope Tag) Monoclonal Antibody (HRP) (CAT# NBAB-105730) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP).

Product Category：Mouse MAb

Reactivity：other

Specificity：Recognize N-terminal and C-terminal Flag Tag in fusion proteins

Immunogen：A synthetic peptide corresponding to the flag-tag sequence (DYKDDDDK).

Isotype：Monoclonal Mouse IgG2b

Purification：Protein A

Buffer：0.2 μm filtered solution in PBS

Conjugate：HRP

Applications：ELISA

Dilution：ELISA: 0.1-1 μg/ml

Storage：This liquid antibody can be stored at 2℃-8℃ for at least two weeks. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or antibody diluent, the antibody is stable for at least two weeks at 2-4℃.