Catalog No.	KAV00302
Product name	Anti-SARS-CoV-2 RBD (BA.5) Human IgA ELISA Kit
Description	PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 RBD (BA.5) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 RBD (BA.5) Human IgA present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgA antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 RBD (BA.5) Human IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications	Used for the quantitative determination of Anti-SARS-CoV-2 RBD (BA.5) Human IgA concentration in serum and plasma.
Stability and Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method	Colorimetric
Precision	Intra-Assay Precision (Precision within an assay): <10% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <15% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.   Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/mL) 33.4 11.3 2.5 36.2 11.9 2.6 Standard deviation 2.1 0.4 0.1 2.4 0.5 0.1 CV (%) 6.2 3.6 3.8 6.6 3.8 4.2
Sample type	Plasma, Serum
Assay type	Quantitative
Sensitivity	0.5 ng/mL
Range	1.56 - 100 ng/mL
Recovery	80-120%
Background	/
Alternative Names	/
Note	For Research Use Only.

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