Catalog No.	KDE16602
Product name	Ramucirumab ELISA Kit
Description	PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD309 has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Ramucirumab in the sample competitively binds to the pre-coated protein with biotin-labeled Ramucirumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Ramucirumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications	Used for the quantitative determination of Ramucirumab concentration in serum and plasma.
Stability and Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Detection method	Colorimetric
Precision	Intra-Assay Precision (Precision within an assay): <20% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <20% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.   Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/mL) 5523.9 1358.9 272.9 5175.1 1340.9 309.4 Standard deviation 615.9 106.4 31.4 448.5 156.6 42.6 CV (%) 11.2 7.8 11.5 8.7 11.7 13.8
Sample type	Plasma, Serum
Assay type	Quantitative
Sensitivity	80.76 ng/mL
Range	156.25 - 10,000 ng/mL
Recovery	80-120%
Background	Ramucirumab is a human monoclonal antibody (IgG1) against vascular endothelial growth factor receptor 2 (VEGFR2), a type II transmembrane tyrosine kinase receptor expressed on endothelial cells. By binding to VEGFR2, Ramucirumab prevents binding of its ligands (VEGF-A, VEGF-C, and VEGF-D), thereby preventing VEGF-stimulated receptor phosphorylation and downstream ligand-induced proliferation, permeability, and migration of human endothelial cells.
Alternative Names	1121B, IMC-1121B, LY3009806, CAS: 15966-93-5
Note	For Research Use Only.

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