Design of the EL4-hCD20-LZ cell line

The EL-4 cell line was established from a C57BL/6 murine lymphoma. These cells form tumors in syngeneic models, upon subcutaneous and systemic (i.v.) injection, in immunocompetent mice.

We generated an EL4-hCD20-LZ clonal cell line expressing high levels of human CD20 (MS4A1 NCBI GeneID: 931), and an optimized dual fusion reporter luciferase-ZsGreen (LZ). EL4-hCD20-LZ cells form tumors in vivo when injected systemically. Luciferase-ZsGreen (LZ) control cells are available.

EL4-hCD20-LZ features

• Express a fully human CD20
• Express a luciferase-ZsGreen (LZ) fusion reporter
• Form tumors and impact survival upon systemic injection in fully immunocompetent mice
• Luciferase from injected cells can be detected by in vivo imaging