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Mouse Cytokine Array GS10
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原料试剂技术服务 动物实验室研发实验室
价格
14152.32
品牌 瑞博奥/RayBio
地区 -
货号 GSM-CYT-10-1
产地 进口
选择规格
16样本
易享客服
RayBio多因子检测
瑞博奥(广州)生物科技股份有限公司
中国广东省广州市
营业执照已审核
RayBio是一家总部位于美国乔治亚州的生物技术公司,专注于开发和制造高质量的蛋白质检测工具以及提供相应的服务,聚焦于蛋白质组学的检测分析领域和多组学的应用,以严格的质量控制和创新的技术为基础。 Raybio具有可用于生命科学研究、疾病研究及疾病治疗相关药物、医学技术等功能探究的全线产品,包括国际领先、最多专利、最多指标的抗体芯片、流式多因子芯片等多因子检测试剂盒超700余种;单靶标检的ELISA试剂盒,可用于细胞因子含量、药物作用等检测;以及超过18,000种抗体、小分子产品,可用于WB、ELISA、IF等免疫学实验。 我们的产品和服务被全球的学术、政府和制药实验室广泛使用,用于疾病诊断、药物筛选和基础研究,已助力全球客户在Nature, Nature Medicine, Cell, Lancet, PNAS等世界顶级期刊上发表了数万篇文章。
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产品规格 图文详情 技术文档
产品规格
品牌名称
瑞博奥/RayBio
货号
GSM-CYT-10-1
国产/进口
进口
规格
16样本
图文详情

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
ADAM-9
  • Gene Symbol: Adam9 Kiaa0021 Mdc9 Mltng
  • Gene ID: Adam9 Kiaa0021 Mdc9 Mltng
  • Accession #: Q61072
CD14
CD39L3
(ENTPD3)
CDNF
Cripto-1
  • Gene Symbol: Tdgf1 Cripto
  • Gene ID: Tdgf1 Cripto
  • Accession #: P51865
CXCL14
(BRAK)
Epimorphin
(Syntaxin 2)
Erythropoietin R
Flt-3
(Flk-2)
Follistatin
Follistatin-like 1
Frizzled-1
Frizzled-4
GDF-3
GDF-7
(BMP-12)
GFR alpha-3
IFN-gamma R2
IL-1 R1
  • Gene Symbol: Il1r1 Il-1r1 Il1ra
  • Gene ID: 16177
  • Accession #: P13504
IL-1 R2
  • Gene Symbol: Il1r2 Il-1r2 Il1rb
  • Gene ID: Il1r2 Il-1r2 Il1rb
  • Accession #: P27931
IL-10 R beta
IL-11 R alpha
IL-15 R alpha
IL-17 RA
IL-17 RC
IL-20 R beta
IL-21 R
IL-23 R
IL-28A
(IFN-lambda 2)
IL-36 beta
(IL-1 F8)
JAM-C
(CD323)
Klotho beta
Laminin alpha 4
Layilin
LDL R
LIF
Lymphotoxin beta R
(TNF RIII)
Matrilin-3
Nephrin
Neurocan
NKp46
(NCR1)

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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