瑞博奥/RayBioHuman, Mouse and Rat Phospho-PDK1 (Ser241) and Total PDK1 ELISAPEL-PDK1-S241-T-1瑞博奥,瑞博奥生物-易享

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Human, Mouse and Rat Phospho-PDK1 (Ser241) and Total PDK1 ELISA

原料试剂技术服务动物实验室研发实验室

价格

21072

品牌瑞博奥/RayBio

地区 -

货号 PEL-PDK1-S241-T-1

产地进口

选择规格

1 x 96-Well Strip Microplate Kit

易享客服

RayBio多因子检测

瑞博奥（广州）生物科技股份有限公司

中国广东省广州市

营业执照已审核

RayBio是一家总部位于美国乔治亚州的生物技术公司，专注于开发和制造高质量的蛋白质检测工具以及提供相应的服务，聚焦于蛋白质组学的检测分析领域和多组学的应用，以严格的质量控制和创新的技术为基础。 Raybio具有可用于生命科学研究、疾病研究及疾病治疗相关药物、医学技术等功能探究的全线产品，包括国际领先、最多专利、最多指标的抗体芯片、流式多因子芯片等多因子检测试剂盒超700余种；单靶标检的ELISA试剂盒，可用于细胞因子含量、药物作用等检测；以及超过18,000种抗体、小分子产品，可用于WB、ELISA、IF等免疫学实验。我们的产品和服务被全球的学术、政府和制药实验室广泛使用，用于疾病诊断、药物筛选和基础研究，已助力全球客户在Nature, Nature Medicine, Cell, Lancet, PNAS等世界顶级期刊上发表了数万篇文章。

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产品规格图文详情技术文档

产品规格

品牌名称

瑞博奥/RayBio

货号

PEL-PDK1-S241-T-1

国产/进口

进口

规格

1 x 96-Well Strip Microplate Kit

图文详情

Product Features

Rapidly measure phosphorylated protein in lysates
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Anti-Phospho Antibody
HRP-Conjugated Secondary Antibody
Assay Diluent
TMB One-Step Substrate
Stop Solution
Lysis Buffer
Positive Control Sample

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Prepare all reagents and samples as instructed in the manual.
Add 100 µl of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 µl of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Typical Data

Positive Control

HT29 cells were treated with Calyculin A. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.

Calyculin A Stimulation of HT29 Cell Line

HT29 cells were treated with Calyculin A. Cell lysates were treated or untreated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot.

Storage/Stability

Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment.

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