瑞博奥/RayBioHuman VEGF IQELISA KitIQH-VEGF-5瑞博奥,瑞博奥生物-易享

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Human VEGF IQELISA Kit

原料试剂技术服务动物实验室研发实验室

价格

21072

品牌瑞博奥/RayBio

地区 -

货号 IQH-VEGF-5

产地进口

选择规格

5 x 96-Well Strip Microplate Kit

易享客服

RayBio多因子检测

瑞博奥（广州）生物科技股份有限公司

中国广东省广州市

营业执照已审核

RayBio是一家总部位于美国乔治亚州的生物技术公司，专注于开发和制造高质量的蛋白质检测工具以及提供相应的服务，聚焦于蛋白质组学的检测分析领域和多组学的应用，以严格的质量控制和创新的技术为基础。 Raybio具有可用于生命科学研究、疾病研究及疾病治疗相关药物、医学技术等功能探究的全线产品，包括国际领先、最多专利、最多指标的抗体芯片、流式多因子芯片等多因子检测试剂盒超700余种；单靶标检的ELISA试剂盒，可用于细胞因子含量、药物作用等检测；以及超过18,000种抗体、小分子产品，可用于WB、ELISA、IF等免疫学实验。我们的产品和服务被全球的学术、政府和制药实验室广泛使用，用于疾病诊断、药物筛选和基础研究，已助力全球客户在Nature, Nature Medicine, Cell, Lancet, PNAS等世界顶级期刊上发表了数万篇文章。

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产品规格图文详情技术文档

产品规格

品牌名称

瑞博奥/RayBio

货号

IQH-VEGF-5

国产/进口

进口

规格

5 x 96-Well Strip Microplate Kit

图文详情

Introduction

The RayBio^{^®} Immuno Qunatitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The RayBio^{^®} Human VEGF IQELISA™ kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of human VEGF in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human VEGF coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.

Standard Curves

Typical Data

Spiking & Recovery Results

Serum spike tests show recovery is 93.3% with a range of 89.7 to 98.0%

Application Notes

Kit Components

VEGF Microplate (Item A)**: 96 well PCR plate coated with anti-human VEGF
Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
Standards (Item C): 2 vials of recombinant human VEGF
Assay Diluent B (Item E): 15 ml of 5x concentrated buffer
Detection Affinity Reagent for VEGF (Item F): 2 vials of a 4x concentrated solution of anti-human VEGF affinity reagent
Primer Solution (Item G): 1.7 ml vial
PCR Master Mix (Item H): 1.2 ml vial
PCR Preparation buffer (Item I): 1 ml vial of 10x concentrated buffer
Final Wash Buffer (Item J): 10 ml vial of 10x concentrated buffer

Other Materials Required

Real-time PCR instrument, Bio-Rad recommended
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for data analysis
Tubes to prepare standard or sample dilutions
Heating block or water bath capable of 80°C

Protocol Outline

Prepare all reagents, samples and standards as instructed
Add 25 µl standard or sample to each well. Incubate for 1.5 - 2.5 hours at room temperature
Add 25 µl detection antibody to each well. Incubate 1 hour at room temperature
Add 15µL Primer solution to each well
Add 10µL of PCR Master Mix to each well
Run real-time PCR

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

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