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收藏Western blot analysis of extracts of various cell lines, using STK4 antibody (144-08043) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot analysis of extracts of various cells, using STK4 antibody (144-08043) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using STK4 antibody (144-08043) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using STK4 antibody (144-08043) at dilution of 1:100 (40x lens).