瑞博奥/RayBioAnti-PUF60 Antibody144-06709瑞博奥,瑞博奥生物-易享

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Anti-PUF60 Antibody

PUF60 Polyclonal Antibody

原料试剂动物实验室研发实验室

价格

询价

品牌瑞博奥/RayBio

地区 -

货号 144-06709

产地进口

选择规格

100 µL

20 µL

易享客服

RayBio多因子检测

瑞博奥（广州）生物科技股份有限公司

中国广东省广州市

营业执照已审核

RayBio是一家总部位于美国乔治亚州的生物技术公司，专注于开发和制造高质量的蛋白质检测工具以及提供相应的服务，聚焦于蛋白质组学的检测分析领域和多组学的应用，以严格的质量控制和创新的技术为基础。 Raybio具有可用于生命科学研究、疾病研究及疾病治疗相关药物、医学技术等功能探究的全线产品，包括国际领先、最多专利、最多指标的抗体芯片、流式多因子芯片等多因子检测试剂盒超700余种；单靶标检的ELISA试剂盒，可用于细胞因子含量、药物作用等检测；以及超过18,000种抗体、小分子产品，可用于WB、ELISA、IF等免疫学实验。我们的产品和服务被全球的学术、政府和制药实验室广泛使用，用于疾病诊断、药物筛选和基础研究，已助力全球客户在Nature, Nature Medicine, Cell, Lancet, PNAS等世界顶级期刊上发表了数万篇文章。

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产品规格图文详情技术文档

产品规格

品牌名称

瑞博奥/RayBio

货号

144-06709

国产/进口

进口

规格

100 µL,20 µL

图文详情

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50

Format

Calculated MW

54kDa/55kDa/57kDa/58kDa/59kDa

Observed MW

65kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PUF60 antibody (144-06709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PUF60 antibody (144-06709) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using PUF60 antibody (144-06709). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug PUF60 antibody (144-06709). Western blot was performed from the immunoprecipitate using PUF60 antibody (144-06709) at a dilition of 1:500.

Expiration:

12 months from the date of shipment when stored properly.

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