Synonyms
BAP antibody, BCAP37 antibody, Bap37 antibody, MGC117268 antibody, PNAS-141 antibody, REA antibody, p22 antibody, PHB2 antibody, B-cell receptor-associated protein BAP37 antibody, prohibitin-2 antibody, B-cell associated protein antibody, D-prohibitin antibody, repressor of estrogen receptor activity antibody, prohibitin 2 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 45 and 299 of Prohibitin 2 (Uniprot ID# Q99623)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Prohibitin 2 antibody detects PHB2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Prohibitin 2 antibody (107-10317) dilution: 1:500.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10317 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA (107-10317) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
107-10317 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.