Synonyms
DCRRP antibody, HPDHASE antibody, HSA250303 antibody, PVIARL antibody, SDR29C1 antibody, TERP antibody, PECR antibody, pVI-ARL antibody, "2,4-dienoyl-CoA reductase-related protein antibody", DCR-RP antibody, "short chain dehydrogenase/reductase family 29C, member 1 antibody", putative short chain alcohol dehydrogenase antibody, peroxisomal trans-2-enoyl-CoA reductase antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 241 and 303 of PECR (Uniprot ID# Q9BY49)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using PECR(107-11003) antibody at 1:200 dilution.

PECR antibody [C2C3], C-term detects PECR protein at peroxisome and cytoplasm on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
PECR antibody [C2C3], C-term (107-11003) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
12% SDS PAGE
107-11003 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.