Synonyms
FLJ46318 antibody, FLJ90018 antibody, LTBP-4 antibody, LTBP4L antibody, LTBP4S antibody, LTBP4 antibody, latent-transforming growth factor beta-binding protein 4 antibody, latent transforming growth factor-beta binding protein 4L antibody, latent transforming growth factor beta binding protein 4 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.01%thimerosal in PBS
Calculated Molecular Weight
173 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 101 and 323 of LTBP4 (Uniprot ID# Q8N2S1)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using LTBP4(107-10281) antibody at 1:200 dilution.

LTBP4 antibody [N1N2], N-term detects LTBP4 protein at cytosol on Adrenal Gland Tumor by immunohistochemical analysis.
Sample: Paraffin-embedded Adrenal Gland Tumor.
LTBP4 antibody [N1N2], N-term (107-10281) dilution: 1:250.

LTBP4 antibody [N1N2], N-term detects LTBP4 protein by Western blot analysis.
A. 30 µg K562 whole cell lysate/extract
5 % SDS-PAGE
LTBP4 antibody [N1N2], N-term (107-10281) dilution: 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10281 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.