瑞博奥/RayBioAnti-LC3 (APG8B) (N-term) Antibody102-13634瑞博奥,瑞博奥生物-易享

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Anti-LC3 (APG8B) (N-term) Antibody

Rabbit Anti-LC3 (APG8B) (N-term) Antibody, 200 µl

原料试剂动物实验室研发实验室

价格

3841

品牌瑞博奥/RayBio

地区 -

货号 102-13634

产地进口

选择规格

200 µl

易享客服

RayBio多因子检测

瑞博奥（广州）生物科技股份有限公司

中国广东省广州市

营业执照已审核

RayBio是一家总部位于美国乔治亚州的生物技术公司，专注于开发和制造高质量的蛋白质检测工具以及提供相应的服务，聚焦于蛋白质组学的检测分析领域和多组学的应用，以严格的质量控制和创新的技术为基础。 Raybio具有可用于生命科学研究、疾病研究及疾病治疗相关药物、医学技术等功能探究的全线产品，包括国际领先、最多专利、最多指标的抗体芯片、流式多因子芯片等多因子检测试剂盒超700余种；单靶标检的ELISA试剂盒，可用于细胞因子含量、药物作用等检测；以及超过18,000种抗体、小分子产品，可用于WB、ELISA、IF等免疫学实验。我们的产品和服务被全球的学术、政府和制药实验室广泛使用，用于疾病诊断、药物筛选和基础研究，已助力全球客户在Nature, Nature Medicine, Cell, Lancet, PNAS等世界顶级期刊上发表了数万篇文章。

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产品规格

品牌名称

瑞博奥/RayBio

货号

102-13634

国产/进口

进口

规格

200 µl

图文详情

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Specificity

Predicted Reactivity

Bovine

Other Names

MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta

NCBI Accession #

NP_073729.1

Other Accession #

A6NCE7; O41515

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14688

Recommended Dilutions

WB: 1:1000; IF: 1:100; IHC: 1:50-100

	Western blot analysis of anti-LC3 (APG8b) in untreated or treated Hela cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
	Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term)(Cat# 102-13634). U251 cells(right) were treated with Chloroquine (50 µM,16h). 102-13634 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
	Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B) (N-term)(Cat# 102-13634). U251 cells(right) were treated with Chloroquine (50 µM,16h). 102-13634 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
	Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term) (Cat# 102-13634). U251 cells(right) were treated with Chloroquine (50 µM,16h). 102-13634 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
	Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
	Western blot analysis of anti-LC3 (APG8b) Pab (Cat# 102-13634) in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
	Western blot analysis of anti-LC3 (APG8b) Pab (Cat# 102-13634) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified Pab.