Introduction
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.
Specificity
Predicted Reactivity
Zebrafish, Bovine
Other Names
CSE1L; CAS; XPO2; Exportin-2; Cellular apoptosis susceptibility protein; Chromosome segregation 1-like protein; Importin-alpha re-exporter
NCBI Accession #
NP_001243064.1;NP_001307.2
Other Accession #
Q9ERK4; Q7SZC2; A5D785
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
110417
Recommended Dilutions
WB: 1:1000
 | Western blot analysis of lysates from Hela, Jurkat, Ramos, mouse NIH/3T3 cell line (from left to right), using CSE1L Antibody (Cat# 102-14253). 102-14253 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
 | Western blot analysis of anti-CSE1L Pab (Cat# 102-14253) in, from left to right, A375, CEM, and mouse heart cell line lysates (35ug/lane). CSE1L(arrow) was detected using the purified Pab. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.