Introduction
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.
Specificity
Predicted Reactivity
Monkey
Other Names
RAD9A; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A
NCBI Accession #
NP_001230153.1;NP_004575.1
Other Accession #
Q4R5X9; NP_004575.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
42547
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | Rad9 Antibody (pS387) (Cat# 102-11127) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Rad9 antibody detected the Rad9 protein (arrow). |
 | Rad9 Antibody (S387) (Cat# 102-11127)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Rad9 Antibody (S387) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of Rad9 Antibody (S387)(Cat# 102-11127) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.