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收藏细胞基因敲除效率:100%
ZDHHC23 NCBI Gene ID:254887
ZDHHC23 Ensembl ID:ENSG00000184307
ZDHHC23 Uniprot ID: Q8IYP9
ZDHHC23基因介绍:Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates and be involved in a variety of cellular processes (Probable). Palmitoyltransferase that mediates palmitoylation of KCNMA1, regulating localization of KCNMA1 to the plasma membrane. May be involved in NOS1 regulation and targeting to the synaptic membrane.
细胞生长培养基:MEM(含NEAA)+10μg/mL Insulin+10% FBS+1% P/S
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/3 传代
细胞倍增时间:~30-72 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。