细胞基因敲除效率：100%

caspase11 NCBI Gene ID：12363

caspase11 Ensembl ID：ENSMUSG00000033538

caspase11 Uniprot ID： P70343

caspase11基因介绍：Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis (PubMed:22002608, PubMed:23348507, PubMed:23887873, PubMed:24031018, PubMed:25119034, PubMed:30135078, PubMed:37001519, PubMed:38632402). Also indirectly activates the NLRP3 and NLRP6 inflammasomes (PubMed:26320999, PubMed:30392956, PubMed:37001519). Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS and GSDMD (PubMed:26375003, PubMed:28314590, PubMed:30392956, PubMed:38632402).

细胞生长培养基：RPMI1640培养基+10%胎牛血清+1%青霉素/链霉素双抗+0.01mMβ巯基乙醇

细胞培养条件：37℃,5% CO2 的培养箱，1/2 到 1/4 传代

细胞倍增时间：~24-48 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell line；Sanger 测序结果显示KO Cell line敲除效率100%。

细胞应用：高敲除效率的基因敲除细胞系（KO Cell line），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。