已关闭
收藏细胞基因敲除效率:100%
TBC1D32 NCBI Gene ID:221322
TBC1D32 Ensembl ID:ENSG00000146350
TBC1D32 Uniprot ID: Q96NH3
TBC1D32基因介绍:Required for high-level Shh responses in the developing neural tube. Together with CDK20, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to Shh signaling (By similarity).
细胞生长培养基:DMEM+10% FBS+1% P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/3到 1/4 传代
细胞倍增时间:~24-36 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。