细胞基因敲除效率：100%

Piezo1 NCBI Gene ID：234839

Piezo1 Ensembl ID：ENSMUSG00000014444

Piezo1 Uniprot ID： E2JF22

Piezo1基因介绍：Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel (PubMed:20813920, PubMed:22343900, PubMed:38184690). Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium (PubMed:20813920, PubMed:22343900, PubMed:38228630). Conductance to monovalent alkali ions is highest for K+, intermediate for Na+ and lowest for Li+. Divalent ions except for Mn2+ permeate the channel but more slowly than the monovalent ions and they also reduce K+ currents (By similarity).

细胞生长培养基：DMEM+10%FBS+1%P,S

细胞培养条件：37℃,5% CO2 的培养箱，1/2 到 1/4 传代

细胞倍增时间：~24-36 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell line；Sanger 测序结果显示KO Cell line敲除效率100%。

细胞应用：高敲除效率的基因敲除细胞系（KO Cell line），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。