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收藏细胞基因敲除效率:100%
CES3 NCBI Gene ID:/
CES3 Ensembl ID:ENSMUSG00000129856
CES3 Uniprot ID: Q8VCT4
CES3基因介绍:Major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters (PubMed:15220344). Hydrolyzes retinyl esters (By similarity).
细胞生长培养基:DMEM+10%FBS+1%P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/4 传代
细胞倍增时间:~24-36 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。