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收藏细胞基因敲除效率:100%
HADH NCBI Gene ID:3033
HADH Ensembl ID:ENSG00000138796
HADH Uniprot ID: Q16836
HADH基因介绍:Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (PubMed:10231530, PubMed:11489939, PubMed:16725361). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (By similarity).
细胞生长培养基:DMEM+10% FBS+1% P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/3 到 1/4 传代
细胞倍增时间:~16 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。