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收藏细胞基因敲除效率:100%
EPCAM NCBI Gene ID:4072
EPCAM Ensembl ID:ENSG00000119888
EPCAM Uniprot ID: P16422
EPCAM基因介绍:May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
细胞生长培养基:DMEM+10% FBS+1% P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/3到 1/4 传代
细胞倍增时间:~24-45 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。