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收藏细胞基因敲除效率:100%
RNF115 NCBI Gene ID:27246
RNF115 Ensembl ID:ENSG00000265491
RNF115 Uniprot ID: Q9Y4L5
RNF115基因介绍:E3 ubiquitin-protein ligase that mediates E2-dependent, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates and may play a role in diverse biological processes. Through their polyubiquitination, may play a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4.
细胞生长培养基:RPMI-1640+10% FBS+0.05mM β-mercaptoethanol+1% P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/4 传代
细胞倍增时间:~24-36 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。