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收藏细胞基因敲除效率:100%
KIAA1712 NCBI Gene ID:80817
KIAA1712 Ensembl ID:ENSG00000164118
KIAA1712 Uniprot ID: Q9C0F1
KIAA1712基因介绍:Centriole-enriched microtubule-binding protein involved in centriole biogenesis. In collaboration with CEP295 and POC1B, is required for the centriole-to-centrosome conversion by ensuring the formation of bona fide centriole wall (PubMed:32060285). Functions as a linker component that maintains centrosome cohesion. Associates with CROCC and regulates its stability and localization to the centrosome (PubMed:31974111).
细胞生长培养基:DMEM+10%FBS+1%p,s
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/4 传代
细胞倍增时间:~25-48 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。