细胞基因敲除效率：100%

TNIK NCBI Gene ID：23043

TNIK Ensembl ID：ENSG00000154310

TNIK Uniprot ID： Q9UKE5

TNIK基因介绍：Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.

细胞生长培养基：DMEM＋10% FBS＋1% P/S

细胞培养条件：37℃,5% CO2 的培养箱，1/3 到 1/4传代

细胞倍增时间：~24-45 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell line；Sanger 测序结果显示KO Cell line敲除效率100%。

细胞应用：高敲除效率的基因敲除细胞系（KO Cell line），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。