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收藏细胞基因敲除效率:100%
MAGEA1 NCBI Gene ID:4100
MAGEA1 Ensembl ID:ENSG00000198681
MAGEA1 Uniprot ID: P43355
MAGEA1基因介绍:May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
细胞生长培养基:RPMI-1640+10% FBS+1% P/S+1%谷氨酰胺+1%丙酮酸钠
细胞培养条件:37℃,5% CO2 的培养箱,1/3 到 1/4传代
细胞倍增时间:~38 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell line;Sanger 测序结果显示KO Cell line敲除效率100%。
细胞应用:高敲除效率的基因敲除细胞系(KO Cell line),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。