细胞基因敲除效率：92%

NDUFS3 NCBI Gene ID：4722

NDUFS3 Ensembl ID：ENSG00000213619

NDUFS3 Uniprot ID：O75489

NDUFS3 基因介绍：Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis (PubMed:12611891). Complex I functions in the transfer of electrons from NADH to the respiratory chain (By similarity). The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

细胞生长培养基：IMDM＋10% FBS＋1% P/S

细胞培养条件：37℃,5% CO2 的培养箱，1/3 到 1/5 传代

细胞倍增时间：~16 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。