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收藏细胞基因敲除效率:>70%
AP1S1 NCBI Gene ID:1174
AP1S1 Ensembl ID:ENSG00000106367
AP1S1 Uniprot ID:P61966
AP1S1 基因介绍:Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
细胞生长培养基:IMDM+10% FBS+1% P/S
细胞培养条件:37℃,5% CO2 的培养箱,1/3 到 1/5 传代
细胞倍增时间:~16 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。