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收藏细胞基因敲除效率:>70%
ACE NCBI Gene ID:1636
ACE Ensembl ID:ENSG00000159640
ACE Uniprot ID:P12821
ACE 基因介绍:Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
细胞生长培养基:IMDM+10% FBS+1% P/S
细胞培养条件:37℃,5% CO2 的培养箱,1/3 到 1/5 传代
细胞倍增时间:~16 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。