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收藏细胞基因敲除效率:>70%
CASP2 NCBI Gene ID:835
CASP2 Ensembl ID:ENSG00000106144
CASP2 Uniprot ID:P42575
CASP2 基因介绍:Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).
细胞生长培养基:McCoy’s 5A+10% FBS+1% P/S
细胞培养条件:37℃,5% CO2 的培养箱,1/3 到 1/4 传代
细胞倍增时间:~25-48 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。