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收藏细胞基因敲除效率:>70%
BMPR1A NCBI Gene ID:657
BMPR1A Ensembl ID:ENSG00000107779
BMPR1A Uniprot ID:P36894
BMPR1A 基因介绍:On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.
细胞生长培养基:McCoy’s 5A+10% FBS+1% P/S
细胞培养条件:37℃,5% CO2 的培养箱,1/3 到 1/4 传代
细胞倍增时间:~25-48 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。