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收藏细胞基因敲除效率:100%
ARAF NCBI Gene ID:369
ARAF Ensembl ID:ENSG00000078061
ARAF Uniprot ID:P10398
ARAF 基因介绍:[Isoform 2]: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.||Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
细胞生长培养基:McCoy’s 5A+10% FBS+1% P/S
细胞培养条件:37℃,5% CO2 的培养箱,1/3 到 1/4 传代
细胞倍增时间:~25-48 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。