细胞基因敲除效率：>70%

GRK6 NCBI Gene ID：2870

GRK6 Ensembl ID：ENSG00000198055

GRK6 Uniprot ID：P43250-2

GRK6 基因介绍：Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

细胞生长培养基：MEM+10%FBS+100U/ml Penicillin-Strep

细胞培养条件：37℃,5% CO2 的培养箱，1/2 到 1/3 传代

细胞倍增时间：~22 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。