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收藏细胞基因敲除效率:92%
EML4 NCBI Gene ID:27436
EML4 Ensembl ID:ENSG00000143924
EML4 Uniprot ID:Q9HC35
EML4 基因介绍:Essential for the formation and stability of microtubules (MTs) (PubMed:16890222, PubMed:31409757). Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs (PubMed:25789526). Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression (PubMed:25789526).
细胞生长培养基:MEM+10%FBS+100U,ml Penicillin-Strep
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/3 传代
细胞倍增时间:~22 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。