细胞基因敲除效率：96%

LMBR1L NCBI Gene ID：55716

LMBR1L Ensembl ID：ENSG00000139636

LMBR1L Uniprot ID：Q6UX01

LMBR1L 基因介绍：Plays an essential role in lymphocyte development by negatively regulating the canonical Wnt signaling pathway (By similarity). In association with UBAC2 and E3 ubiquitin-protein ligase AMFR, promotes the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 (By similarity). LMBR1L stabilizes the beta-catenin destruction complex that is required for regulating CTNNB1 levels (By similarity). Acts as a LCN1 receptor and can mediate its endocytosis (PubMed:11287427, PubMed:12591932, PubMed:23964685).

细胞生长培养基：RPMI-1640＋10% FBS＋1% P,S

细胞培养条件：37℃,5% CO2 的培养箱，1/2到 1/4 传代

细胞倍增时间：~17 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。