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收藏细胞基因敲除效率:100%
NEK10 NCBI Gene ID:152110
NEK10 Ensembl ID:ENSG00000163491
NEK10 Uniprot ID:Q6ZWH5
NEK10 基因介绍:Plays a role in the cellular response to UV irradiation. Mediates G2/M cell cycle arrest, MEK autoactivation and ERK1/2-signaling pathway activation in response to UV irradiation. In ciliated cells of airways, it is involved in the regulation of mucociliary transport (PubMed:31959991).
细胞生长培养基:MEM(含NEAA)+10μg/mL Insulin+10% FBS+1% P/S
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/3 传代
细胞倍增时间:~30-72 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。