细胞基因敲除效率：87%

KIF5B NCBI Gene ID：3799

KIF5B Ensembl ID：ENSG00000170759

KIF5B Uniprot ID：P33176

KIF5B 基因介绍：Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).

细胞生长培养基：DMEM＋10% FBS＋1% P/S

细胞培养条件：37℃,5% CO2 的培养箱，1/2 到 1/4 传代

细胞倍增时间：~36-72 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。