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收藏细胞基因敲除效率:>70%
IL13 NCBI Gene ID:3596
IL13 Ensembl ID:ENSG00000169194
IL13 Uniprot ID:P35225
IL13 基因介绍:Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages (By similarity).
细胞生长培养基:RPMI-1640+10% FBS+1% P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/3到 1/4 传代
细胞倍增时间:~24-36 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。