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收藏细胞基因敲除效率:>70%
FASN NCBI Gene ID:2194
FASN Ensembl ID:ENSG00000169710
FASN Uniprot ID:P49327
FASN 基因介绍:Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
细胞生长培养基:RPMI-1640+10% FBS+1% P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/2到 1/4 传代
细胞倍增时间:~30 h
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。