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收藏细胞基因敲除效率:82%
Hyal2 NCBI Gene ID:15587
Hyal2 Ensembl ID:ENSMUSG00000010047
Hyal2 Uniprot ID:O35632
Hyal2 基因介绍:Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R (By similarity).
细胞生长培养基:DMEM+15%FBS+1%双抗
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/4 传代
细胞倍增时间:~24-36 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。