细胞基因敲除效率：100%

TP53 NCBI Gene ID：/

TP53 Ensembl ID：ENSMUSG00000054226

TP53 Uniprot ID：P70399

TP53 基因介绍：Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:15077110, PubMed:15159415, PubMed:20362325, PubMed:20453858, PubMed:23333305, PubMed:26308889). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:20362325, PubMed:23333305, PubMed:30297459).

细胞生长培养基：DMEM+10%FBS+1%P,S

细胞培养条件：37℃,5% CO2 的培养箱，1/2 到 1/4 传代

细胞倍增时间：~16 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。