细胞基因敲除效率：90%

trmt61a NCBI Gene ID：328162

trmt61a Ensembl ID：ENSMUSG00000060950

trmt61a Uniprot ID：Q80XC2

trmt61a 基因介绍：Catalytic subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N1-methyltransferase complex, which mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.

细胞生长培养基：DMEM＋10% FBS＋1% P,S

细胞培养条件：37℃,5% CO2 的培养箱，1/6到 1/8 传代

细胞倍增时间：~12-30 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。