细胞基因敲除效率：100%

Vim NCBI Gene ID：100689099

Vim Ensembl ID：ENSCGRG00015022110

Vim Uniprot ID：P48670

Vim 基因介绍：Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).

细胞生长培养基：MEM（含NEAA）＋10% FBS＋1% P,S

细胞培养条件：37℃,5% CO2 的培养箱，1/2到 1/4 传代

细胞倍增时间：~20-24 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。