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收藏细胞基因敲除效率:100%
LAMC2 NCBI Gene ID:3918
LAMC2 Ensembl ID:ENSG00000058085
LAMC2 Uniprot ID:Q13753
LAMC2 基因介绍:Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.
细胞生长培养基:RPMI-1640:Ham's F-12(1:1)+10% FBS+1%P,S
细胞培养条件:37℃,5% CO2 的培养箱,1/4 到 1/8 传代
细胞倍增时间:~13-25 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。