已关闭
收藏细胞基因敲除效率:100%
myh9 NCBI Gene ID:4627
myh9 Ensembl ID:ENSG00000100345
myh9 Uniprot ID:P35579
myh9 基因介绍:Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping (By similarity). During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed:20052411).
细胞生长培养基:(客户提供)90%ECM+10%FBS
细胞培养条件:37℃,5% CO2 的培养箱,1/2 到 1/4 传代
细胞倍增时间:~24-36 hours
细胞支原体检测结果:阴性
细胞开发路径:采用CRISPR-RNP方法生成稳定KO Cell Pool;Sanger 测序结果显示KO Cell Pool敲除效率>70%
细胞应用:高敲除效率的基因敲除细胞池(KO Cell Pool),特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。