MICAL2 is a 1,124 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one LIM zinc-binding domain and one calponin-homology domain. Using FAD as a cofactor, MICAL2 interacts with Rab 1B, plexin-A4 and Vimentin and, via these interactions, may play a role in cytoskeletal regulation, specifically at intermediate filaments. MICAL2, which exists as two alternatively spliced isoforms, is expressed at high levels in heart and brain and at lower levels in ovary, skeletal muscle, liver, testis and kidney tissue.