细胞基因敲除效率：>70%

MBD2 NCBI Gene ID：8932

MBD2 Ensembl ID：ENSG00000134046

MBD2Uniprot ID：Q9UBB5

MBD2基因介绍：Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

细胞生长培养基：Ham's F-12＋10% FBS＋1% P,S

细胞培养条件：37℃,5% CO2 的培养箱，1/2到 1/4 传代

细胞倍增时间：~20 hours

细胞支原体检测结果：阴性

细胞开发路径：采用CRISPR-RNP方法生成稳定KO Cell Pool；Sanger 测序结果显示KO Cell Pool敲除效率>70%

细胞应用：高敲除效率的基因敲除细胞池（KO Cell Pool），特别适用于初步功能分析、复杂疾病模型的开发、精准药物筛选以及广泛的基因发现研究。